Antifungal susceptibility testing (AFST)

Publications in journals

EUCAST official publications of breakpoints and methods

 

1. Technical notes on susceptibility testing of fungi


EUCAST DEFINITIVE DOCUMENT  E.DEF 9.1: Method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia forming mould (Download)

 

EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST). (Download)

 

EUCAST Definitive Document EDef 7.1: method for the determination of broth dilution MICs of antifungal agents for fermentative yeasts (Download)

 

2. Technical notes on antifungal breakpoints

 

Breakpoints for Candida

Amphotericin B

Voriconazole

Posaconazole

Micafungin, anidulafungin and fluconazole

 

Breakpoints for Aspergillus
Amphotericin B, itraconazole, and posaconazole

Voriconazole

 

3. Other publications

 

Comparison of DMSO and water as solvents for echinocandins susceptibility testing by EUCAST methodology. Alastruey-Izquierdo A, Gómez-López A, Arendrup MC, Lass-Florl C, Hope WW, Perlin DS, Rodriguez-Tudela JL, Cuenca-Estrella M. 2012. J Clin Microbiol. 50:2509-2012

http://jcm.asm.org/content/50/7/2509.long

Caspofungin Etest susceptibility testing of Candida species: risk of misclassification of susceptible isolates of C. glabrata and C. krusei when adopting the revised CLSI caspofungin breakpoints. Arendrup MC, Pfaller MA; Danish Fungaemia Study Group. Antimicrob Agents Chemother. 2012 Jul;56(7):3965-8.

http://aac.asm.org/content/56/7/3965.long


Evaluation of Caspofungin Susceptibility Testing by the New VITEK(R) 2 AST-YS06 Yeast Card Using a Unique Collection of FKS Wild Type and Hot Spot Mutant Isolates Including the Five Most Common Candida Species. Astvad KM, Perlin DS, Johansen HK, Jensen RH, Arendrup MC. Antimicrob Agents Chemother. 2012 57(1):177-82

http://aac.asm.org/content/early/2012/10/16/AAC.01382-12.long


Comparison of the VITEK 2 antifungal susceptibility system with the CLSI and the EUCAST broth microdilution reference methods and with the Sensititre Yeast-One and the Etest techniques for the detection in vitro of antifungal resistance in yeasts. Cuenca-Estrella M, Gomez-Lopez A, Alastruey-Izquierdo A, Bernal-Martinez L, Cuesta I, Buitrago MJ, Rodriguez-Tudela JL. J Clin Microbiol 2010, 48(5):1782.

http://jcm.asm.org/content/48/5/1782.full.pdf+html


Multicenter comparison of the ISO standard 20776-1 and the serial 2-fold dilution procedures to dilute hydrophilic and hydrophobic antifungal agents for susceptibility testing. Gomez-Lopez A, Arendrup MC, Lass-Floerl C, Rodriguez-Tudela JL, Cuenca-Estrella M. J Clin Microbiol 2010, 48(5):1918.

http://jcm.asm.org/content/48/5/1918.full.pdf+html


Echinocandin susceptibility testing of Candida species: comparison of EUCAST E.Def 7.1, CLSI M27-A3, Etest, disk diffusion, and agar dilution methods with RPMI and isosensitest media. Arendrup MC, Garcia-Effron G, Lass-Flörl C, Lopez AG, Rodriguez-Tudela JL, Cuenca-Estrella M, Perlin DS. Antimicrob Agents Chemother 2010; 54: 426-39

http://aac.asm.org/content/54/1/426.full.pdf+html


Evaluation by data mining techniques of fluconazole breakpoints established by the clinical and laboratory standards institute (CLSI) and comparison with those of the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Cuesta I, Bielza C, Cuenca-Estrella M, Larrañaga P, Rodríguez-Tudela JL. Antimicrob Agents Chemother 2010; 54: 1541-6

http://aac.asm.org/content/54/4/1541.full.pdf+html


EUCAST breakpoints for antifungals. Rodriguez-Tudela JL, Arendrup MC, Cuenca-Estrella M, Donnelly JP, Lass-Florl C. Drug News Perspect 2010; 23: 93-7

http://journals.prous.com/journals/servlet/xmlxsl/pk_journals.xml_summaryn_pr?p_JournalId=3&p_RefId=1400855


Association of fluconazole pharmacodynamics with mortality in patients with candidemia. Arendrup MC, Cuenca-Estrella M, Donnelly JP, Lass-Flörl C, Rodriguez-Tudela JL. Antimicrob Agents Chemother 2009; 53: 2704-5

http://aac.asm.org/content/53/6/2704.full.pdf+html

 

Data mining validation of fluconazole breakpoints established by the European Committee on Antimicrobial Susceptibility Testing. Cuesta I, Bielza C, Larrañaga P, Cuenca-Estrella M, Laguna F, Rodriguez-Pardo D, Almirante B, Pahissa A, Rodríguez-Tudela JL. Antimicrob Agents Chemother 2009; 53: 2949-54

http://aac.asm.org/content/53/7/2949.full.pdf+html


Breakpoints for susceptibility testing should not divide wild-type distributions of important target species. Arendrup MC, Kahlmeter G, Rodriguez-Tudela JL, Donnelly JP. Antimicrob Agents Chemother 2009; 53:1628-9.

http://aac.asm.org/content/53/4/1628.full.pdf+html

 

Echinocandin susceptibility testing of Candida species: comparison of EUCAST E.Def 7.1, CLSI M27-A3, Etest, disk diffusion, and agar dilution methods with RPMI and Isosensitest media. Arendrup MC, Garcia-Effron G, Lass-Flörl C, Lopez AG, Rodriguez-Tudela JL, Cuenca-Estrella M, Perlin DS. Antimicrob Agents Chemother 2010;54(1):426-39.

http://aac.asm.org/content/54/1/426.full.pdf+html

Breakpoints for antifungal agents: an update from EUCAST focussing on echinocandins against Candida spp. and triazoles against Aspergillus spp. Arendrup MC, Cuenca-Estrella M, Lass-Flörl C, Hope WW. Drug Res Update 2013;16(6):81-95

http://www.sciencedirect.com/science/article/pii/S1368764614000028


Interlaboratory variability of Caspofungin MICs for Candida spp. Using CLSI and EUCAST methods: should the clinical laboratory be testing this agent? Espinel-Ingroff A, Arendrup MC, Pfaller MA, Bonfietti LX, Bustamante B, Canton E, Chryssanthou E, Cuenca-Estrella M, Dannaoui E, Fothergill A, Fuller J,Gaustad P, Gonzalez GM, Guarro J, Lass-Flörl C, Lockhart SR, Meis JF, Moore CB, Ostrosky-Zeichner L, Pelaez T, Pukinskas SR, St-Germain G, Szeszs MW, Turnidge J. Antimicrob Agents Chemother 2013; 57(12): 5836-42.

http://aac.asm.org/cgi/pmidlookup?view=long&pmid=24018263


EUCAST testing of Isavuconazole susceptibility in Aspergillus: comparison of results for Inoculum standardization using Conidium counting versus optical density. Arendrup MC ,Howard S, Lass-Flörl C, Mouton JW, Meletiadis J, Cuenca-Estrella M. Antimicrob Agents Chemother 2014;58(11): 6432-6.

http://aac.asm.org/content/58/11/6432.long

 

 

 

Last update: 23 November 2012