Documents

EUCAST publications in journals

Here you will find EUCAST articles in scientific journals.

Whole genome sequencing (WGS) offers the potential to predict antimicrobial susceptibility from a single assay. The European Committee on Antimicrobial Susceptibility Testing established a subcommittee to review the current development status of WGS for bacterial antimicrobial susceptibility testing (AST).

The published evidence for using WGS as a tool to infer antimicrobial susceptibility accurately is currently either poor or non-existent and the evidence / knowledge base requires significant expansion. The primary comparators for assessing genotypic–phenotypic concordance from WGS data should be changed to epidemiological cut-off values in order to improve differentiation of wild-type from non-wild-type isolates (harbouring an acquired resistance). Clinical breakpoints should be a secondary comparator.

BSAC recommends UK laboratories to adopt EUCAST disk diffusion methodology in 2016.

The BSAC Standing Committee on Antimicrobial Susceptibility Testing implemented the EUCAST breakpoints in its own disc diffusion susceptibility testing method as harmonized breakpoints were agreed to over the years. Since the development of the EUCAST disc diffusion method, several countries with their own disc diffusion methods have switched to the EUCAST method, and BSAC will replace support of its own disc diffusion method with support for the EUCAST method from January 2016. The EUCAST breakpoints are also available in automated systems. The harmonized breakpoints and methods will help to avoid different reports of susceptibility for the same isolate in different countries and enable more reliable comparison of resistance rates in surveillance studies in different countries. D Brown, M Wootton and R Howe, Leading article in JAC

EUCAST - the 2014 Garrod lecture.

EUCAST – are we heading towards international agreement?

Journal of Antimicrobial Chemotherapy Advance Access published June 18, 2015.
Gunnar Kahlmeter.
 

EUCAST in 2014 - Eurosurveillance

Widespread implementation of EUCAST breakpoints for antibacterial susceptibility testing in Europe.

Eurosurveillance, Volume 20, Issue 2, 15 January 2015.
D Brown, R Cantón, L Dubreuil, S Gatermann, C Giske, A MacGowan, L Martínez-Martínez, J Mouton, R Skov, M Steinbakk, C Walton, O Heuer, M J Struelens, L Diaz Högberg, G Kahlmeter.
 

EUCAST Disk Diffusion

Development of the EUCAST Disk Diffusion Method for antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories.
E. Matuschek, D. F. J. Brown and G. Kahlmeter. Published in CMI 2013

 

The role of Pk/Pd in setting breakpoints

The role of pharmacokinetics/pharmacodynamics in setting clinical
MIC breakpoints - the EUCAST approach
 (published in CMI 2012)

J. W. Mouton, D. F. J. Brown, P. Apfalter, R. Canton, C. G. Giske, M. Ivanova, A. P. MacGowan, A. Rodloff, C.-J. Soussy, M. Steinbakk and G. Kahlmeter. CMI 2012.

 

EUCAST Expert Rules

EUCAST Expert rules in antimicrobial susceptibility testing.
R. Leclercq, R. Canton, D. F. J. Brown, C. G. Giske, P. Heisig, A. P. MacGowan, J. W. Mouton, P. Nordmann, A. C. Rodloff, G. M. Rossolini, C.-J. Soussy, M. Steinbakk, T. G. Winstanley and G. Kahlmeter. CMI 2012.

 

Arendrup MC, Garcia-Effron G, Lass-Flörl C, Lopez AG, Rodriguez-Tudela JL, Cuenca-Estrella M, Perlin DS. Antimicrob Agents Chemother 2010; 54: 426-39
link to full article

Rodriguez-Tudela JL, Arendrup MC, Cuenca-Estrella M, Donnelly JP, Lass-Florl C. Drug News Perspect 2010; 23: 93-7
link to full article

Arendrup MC, Cuenca-Estrella M, Donnelly JP, Lass-Flörl C, Rodriguez-Tudela JL. Antimicrob Agents Chemother 2009; 53: 2704-5
link to full article

Cuesta I, Bielza C, Larrañaga P, Cuenca-Estrella M, Laguna F, Rodriguez-Pardo D, Almirante B, Pahissa A, Rodríguez-Tudela JL. Antimicrob Agents Chemother 2009; 53: 2949-54
link to full article

Arendrup MC, Kahlmeter G, Rodriguez-Tudela JL, Donnelly JP. Antimicrob Agents Chemother 2009; 53:1628-9.
link to full article

Susceptibility testing of Candida species to echinocandins: comparison of EUCAST EDef 7.1, CLSI M27-A3, Etest, disk diffusion and agar-dilution using RPMI and IsoSensitest medium

Arendrup MC, Garcia-Effron G, Lass-Floerl C, Gomez Lopez A, Rodriguez-Tudela JL, Cuenca-Estrella M, Perlin DS. Antimicrob Agents Chemother. 2009 Nov 2. Epub ahead of print.
Link to full article

Method for the determination of broth dilution of antifungal agents for fermentative yeasts, Apr 2008

Subcommittee on Antifungal Susceptibility Testing (ASFT) of the ESCMID European Committee for Antimicrobial Susceptibility Testing (EUCAST), EUCAST Definitive Document E.DEF 7.1: method for the determination of broth dilution of antifungal agents for fermentative yeasts. CMI 2008; 14:398-405.

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Drobniewski F, Rüsch-Gerdes S, Hoffner S. EUCAST document E.DEF 8.1: antimicrobial susceptibility testing of Mycobacterium tuberculosis - Report of the Subcommittee on Antimicrobial Testing of Mycobacterium tuberculosis of the European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID). CMI 2007; 13:1144-56.

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Chryssanthou E and Cuenca-Estrella M (2006) Comparison of the EUCAST-AFST broth dilution method with the CLSI reference broth dilution method (M38-A) for susceptibility testing of posaconazole and voriconazole against Aspergillus spp.  Clin Microbiol Infect; 12:901-904.

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Kahlmeter G, Brown DFJ, Goldstein FW, et al. Editorial: European Committee on Antimicrobial Susceptibility Testing (EUCAST). CMI 2006; 12:501-503.

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Kahlmeter G, Brown DFJ. (2004) Harmonisation of antimicrobial breakpoints in Europe – can it be achieved?. Clinical Microbiology Newsletter 2004; 26:187-192.

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Kahlmeter G, Brown DFJ, Goldstein FW et al. European harmonization of MIC breakpoints for antimicrobial susceptibility testing of bacteria.
J Antimicrob Chemother 2003; 52:145-148.

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European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society for Clinical Microbiology and Infectious Diseases (ESCMID). EUCAST Discussion Document E. Dis 5.1: determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution. Clin Microbiol Infec 2003; 9 (insert): 1-7.
Link to full document

 

This discussion document was not developed into a EUCAST definitive document because EUCAST was aware of the proposed development of an international (ISO) standard for broth microdilution, which was eventually published in 2006.

European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society for Clinical Microbiology and Infectious Diseases (ESCMID). EUCAST Definitive Document E. Def 2.1: determination of antimicrobial susceptibility test breakpoints. Clin Microbiol Infec 2000; 6:570-572.

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European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society for Clinical Microbiology and Infectious Diseases (ESCMID). EUCAST Definitive Document E. Def 3.1: determination of minimum inhibitory concentrations (MICs) of antibacterial agents by agar dilution. Clin Microbiol Infec 2000; 6:509-515.

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EUCAST Definitive Document E.Def 1.2: terminology relating to methods for the determination of susceptibility of bacteria to antimicrobial agents. Clin Microbiol Infec 2000; 6:503-508.

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