Rapid AST in bloodcultures

Breakpoints validated for EUCAST short incubation disk diffusion directly from positive blood culture bottles.

For interpretation of rapid AST directly from blood culture bottles, reading and interpreting inhibition zones after 4 hours, 6 hours or 8 hours, always use the RAST Breakpoint Tables (see below), never use EUCAST standard 16 - 20 h breakpoints (regular EUCAST breakpoint tables).

 

Breakpoint tables for Rapid AST directly from blood cultue bottles (pdf-file for printing)

Breakpoint tables for Rapid AST directly from blood cultue bottles (Excel-table for screen)

 

QC recommendations to facilitate the introduction of the methodology in the laboratory are embedded in the breakpoint table (QC tab).

Erratum - in the method´s document and in the RAST breakpoint table:
"RAST QC is performed by inoculating blood culture bottles with 1 mL of a 100-200 CFU/mL solution (0.5 McFarland diluted 1:1 000 000 (not as in the document and tables 1:100 000) of the QC strain and with addition of approximately 5 mL sterile blood. The inoculated bottles are incubated in the blood culture instrument and processed according to the described methodology when a positive signal has been obtained." (4 March, 2019)

 

 

 

28 November, 2018