EUCAST Disk Diffusion Test Methodology

The EUCAST disk diffusion test is based on MH media and disks of a good quality. It is calibrated to EUCAST clinical breakpoints using broth microdilution for MIC determination. Updates are published regularly. 

See also EUCAST instruction videos.

• Disk diffusion - Manual v 10.0 (1 January, 2022)
• Disk diffusion - Slide show v 10.0 (1 January, 2022)
• Disk diffusion - Reading guide v 9.0 (1 January 2022)
• Anaerobic bacteria - disk diffusion methodology v 1.0 (1 January 2022) including QC recommendations (the difficulties related to ordering the QC strain C. perfringens DSM 25589 from Germany have been solved - it can now be ordered from DSM and CCUG).
  Disk diffusion breakpoints for anaerobic bacteria are valid for FAA with 5% mechanically defibrinated horse blood as the only additive.
• Anaerobic bacteria - disk diffusion reading guide v 1.0 (1 January 2022) 
  Disk diffusion breakpoints for anaerobic bacteria are valid for FAA with 5% mechanically defibrinated horse blood as the only additive.

For translations to other languages - contact National AST committees (NAC).

For previous versions of documents - see Previous versions.

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