AST of mycobacteria

Antimicrobial susceptibility testing of Mycobacteria

The EUCAST AMST develops breakpoints and methods for antimicrobial susceptibility testing of mycobacteria.

Anyone with an interest in antimicrobial agents in general and antimicrobial breakpoints in particular is invited to contact EUCAST, ESCMID or one of the National Breakpoint Committees. Use the EUCAST subject related contact form.


Remit of the EUCAST AMST subcommittee

  • Draft an SOP for a reference MIC method
  • Define a set of quality control strains
  • Organise the assembly of a set of reference strains to comprise approximately 100 wild type isolates and 50 identified as resistant to one or two agents (but not MDR MTb).
  • Organise studies to establish reproducibility of the reference method in at least three laboratories.
  • Organise preparation of MIC distributions for the reference strains. The reference MICs will be performed in at least three reference laboratories.
  • Interact with the EUCAST Steering Committee in the setting of breakpoints for antimycobacterial agents.
  • Define the relevance of molecular testing and mutation frequencies.


Information for industry aiming to bring agents to EUCAST for review and revision of breakpoints or a new agent to EMA for registration is available at Information for industry


Subcommittee members 2020

Emmanuelle Cambau (chair) France ESGMYC
Thomas Schön  Sweden ESGMYC
Miguel Viveiros Portugal ESGMYC
Miguel Santin Spain ESGMYC
Daniela Cirillo Italy ERLTB-net
Jim Werngren, ESGMYC
Gerard Lina France, EUCAST

European Committee on Antimicrobial Susceptibility Testing (EUCAST)

ESCMID study group for mycobacterial infections (ESGMYC)

European Reference Laboratory Network for Tuberculosis (ERLTB-net)



For Copyright statement and reference to EUCAST material, see the homepage of EUCAST.