AST of bacteria

EUCAST Disk Diffusion Test Methodology

The EUCAST disk diffusion test is based on MH media and disks of a good quality. It is calibrated to EUCAST clinical breakpoints using broth microdilution for MIC determination. Updates are published regularly. 

See also EUCAST instruction videos.

 

Disk diffusion - Manual v 9.0 (1 January, 2021)

Disk diffusion - Slide show v 9.0 (1 January, 2021)

Disk diffusion - Reading guide v 8.0 (1 January, 2021)

 

EUCAST disk diffusion of anaerobic bacteria is under development 2021. Reviewed clinical breakpoints and disk diffusion correlates will be published with breakpoint table v 12.0 (1 January, 2022). The method will be valid for 5 species (Bacteroides spp, Prevotella spp, Fusobacterium necrophorum, Clostridium perfringens and Cutibacterium acnes) and for anaerobic incubation for 16 - 20h (extended incubation not allowed). For anyone who wants to prepare and practice, EUCAST already now publish the methodology, reading guide and QC criteria.

 

For translations to other languages - see Translations.

For previous versions of documents - see Previous versions.

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