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Instruction videos: EUCAST disk diffusion methodology
In collaboration with the World Health Organisation (WHO), EUCAST publishes instruction videos on how to perform antimicrobial susceptibility testing (AST) using EUCAST recommended methods and interpretation.
The videos are published on Youtube™ and have an English speaker voice and subtitles in different languages. Since not all countries may access Youtube™, videos in some languages are made available directly on this page below.
1. Preparation of inoculum
2. Inoculation
3. Application of disk and incubation
4. Reading of zones
5. Guidance on breakpoint table
6. Storage and handling of media and disks
7. Quality control
1. Preparation of inoculum
2. Inoculation
3. Application of disk and incubation
4. Reading of zones
5. Guidance on breakpoint table
The instruction videos from EUCAST on how to perform antimicrobial susceptibility disk diffusion testing was produced by a project team from EUCAST and WHO:
The following are acknowledged for translations of subtitles into languages other than English:
German
Sören Gatermann, Michael Kresken and Axel Hamprecht, all German NAC.
Russian
Roman Kozlov, Marina Sukhorukova, Mikhail Edelstein, all Institute of Antimicrobial Chemotherapy (IAC), Smolensk State Medical University (SSMU).
Turkish
ADTS working Group (Turkish NAC for EUCAST).
French
Luc Dubreuil, Université de Lille, France.
Spanish
Luis Martinez-Martinez and Antonio Oliver, both Spanish Committee of the Antibiogram (Coesant).
Portuguese/Brazilian
Ana Gales and Jorge Sampaio, Brazilian Committee on Antimicrobial Susceptibility Testing (BrCAST).
Arabic
Magda Rakha, Consultant Public Health laboratories and Humayun Asghar, WHO EMRO.
Czech
Pavla Urbaskova, Helena Zemlickova and Vladislav Jakubu, all members of the Czech NAC.
Chinese
Yu Qing Liiu, member of the Chinese NAC, and Hong Yin, clinical microbiology, Falun, Sweden.
Romanian
Olga Burduniuc (Chair of Moldovan NAC)
Explanatory video on the new definitions of S, I and R and on the use of the Area of Technical Uncertainty (ATU) (2019, 22 minutes)
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