Methods in AST of Mycobacterium species
The EUCAST reference method for the Mycobacterium tuberculosis complex is broth microdilution in Middlebrook 7H9.
- EUCAST reference method for MIC determination of Mycobacterium tuberculosis (20 Feb, 2025); Previous version (9 October, 2024)
- Annexe presenting examples of practical and detailed laboratory protocols (20 Feb, 2025).
- SOP for calibrating surrogate methods against the EUCAST reference MIC method.
- Rationale for the reference method for MIC determination of M. tuberculosis.
- Inoculum preparation and performing and CFU counts.
- Consultation from the subcommittee on Antimycobacterial susceptibility testing closed 19 April, 2024. Comments and EUCAST responses available since 7 October, 2024.
Anyone with an interest in antimicrobial agents in general and antimicrobial breakpoints in particular is invited to contact EUCAST, ESCMID or one of the National Breakpoint Committees. Use the EUCAST subject related contact form.
