Methods in AST of Mycobacterium species
The EUCAST reference method for the Mycobacterium tuberculosis complex is broth microdilution in Middlebrook 7H9.
- EUCAST reference method for MIC determination of Mycobacterium tuberculosis.
- Microtiter plate outline (Appendix 1)
- SOP for calibrating surrogate methods against the EUCAST reference MIC method.
- Rationale for the reference method for MIC determination of M. tuberculosis.
- Inoculum preparation and performing and CFU counts.
- Consultation from the subcommittee on Antimycobacterial susceptibility testing on "Reference protocol for MIC determination of anti-tuberculous agents against isolates of the Mycobacterium tuberculosis complex in Middlebrook 7H9 broth".
Consultation period 11 March 2024 - 19 April 2024.