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General

Publications and presentations

Publications and presentations En Route towards European Clinical Breakpoints for Veterinary Antimicrobial Susceptibility Testing: A Position Paper Explaining the VetCAST Approach. P-L Toutain et al. Frontiers in Microbiology, December 2017. Go to publication Population pharmacokinetics of…

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Resistance Detection

Resistance Detection Successful treatment of infectious diseases relies on knowledge and understanding of when antimicrobial resistance is an expected phenotype of the organism, and when resistance to antimicrobial agents may develop. The following documents provide up-to-date advice on how to…

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SOPs

EUCAST SOPs SOP 1.5 Setting breakpoints for new antimicrobial agents (9 May, 2025) Previous versions 1.4 ; 1.3 ; 1.2 ; 1.1 SOP 2.5 Harmonising breakpoints for existing antimicrobial agents (12 May, 2025) Previous versions: 2.4 ; 2.3 ; 2.2 ; 2.1 SOP 3.4 Review and revision of breakpoints (6 May,…

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MIC:Zone Diameter correlations

MIC:Zone Diameter correlations The EUCAST Disk Diffusion test was developed by EUCAST under the auspices of ESCMID and with the help of many laboratories. The help of these laboratories is gratefully acknowledged. Most are listed under the EUCAST laboratory network . The work started in 2009 and is…

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Development of clinical breakpoints and ECOFFs

Development of clinical breakpoints and ECOFFs How to collect and handle PK data Supporting information Guideline VetCAST PK analysis Author: VetCAST Date 06/04/18 Download PDF Guideline to collect, archive, handle and analyse pharmacokinetic data for VetCAST The purpose of this Guideline document…

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National AST Committees (NAC)

National AST Committees (NAC) EUCAST recommends that countries form a “National Antimicrobial Susceptibility Testing Committee” (or a committee corresponding to this description). Developing a NAC in a Country can be useful when adopting or implementing EUCAST antimicrobial susceptibility testing…

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Rapid AST directly from blood culture bottles

Rapid AST directly from blood culture bottles EUCAST has developed a method for rapid AST (reading at 4, 6 or 8h and since April 2022 also after 16-20 hours incubation) directly from positive blood culture bottles (RAST). The rationale is available in JAC . Following the initial development,…

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Reference Method

Reference Method The EUCAST reference method for the Mycobacterium tuberculosis complex is broth microdilution in Middlebrook 7H9. See following documents for further information. EUCAST reference protocol for MIC determination of anti-tuberculous agents against isolates of the Mycobacterium…

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Rationale Documents

Rationale Documents EUCAST rationale documents summarise the information on which the EUCAST clinical breakpoints are based. Bedaquiline Rationale for EUCAST clinical breakpoints Version 1.3, 4 July 2019 Download PDF Bedaquiline (TMC207) is a diarylquinoline, a new class of anti-tuberculosis (TB)…

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Organisation

Organisation The European Committee on Antimicrobial Susceptibility Testing (EUCAST) is mandated by ESCMID, EMA, ECDC, EFSA and partners across Europe to define, harmonize and standardize antimicrobial breakpoints and testing methods. EUCAST structure and procedures have been developed with ESCMID,…

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