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General

Resistance Detection in RAST

Resistance Detection in RAST Some resistance mechanisms require other than clinical breakpoints for sensitive detection. EUCAST has validated using separate screening cut-offs for the detection of E. coli and K. pneumoniae with ESBL or carbapenemases. Screening for resistance mechanisms using RAST…

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Publications and Presentations

Publications and Presentations 2025 2023 2022 2020 Archive The Mycobacterium tuberculosis complex based on the EUCAST reference protocol. Mansjö M, Espinosa-Gongora C, Samanci I, Groenheit R, Werngren J. Antimicrob Agents Chemother. 2025 Feb 13;69(2):e0094624. doi: 10.1128/aac.00946-24. Epub 2024…

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Methods and Quality Control

Methods and Quality Control EUCAST has validated a method for direct plating of disk diffusion MH and MHF agar plates from positive blood culture bottles. The plates are immediately incubated and read after 4, 6, 8 or 16-20 hours (but not beyond) of incubation. Document Link Methodology - EUCAST…

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MIC and Zone Distributions, ECOFFs

The database of MIC and zone diameter distributions was created by Gunnar Kahlmeter for EUCAST from 2002 and onwards. More data is regularly added and all data is curated by Gunnar Kahlmeter and John Turnidge. The website gives MIC distributions and inhibition zone diameter distributions for…

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Rationale documents

Rationale documents Amphotericin B v 3.0 (26 June 2025) Anidulafungin vs. Candida v 5.0 (26 June 2025) Caspofungin vs Candida - there is no caspofungin RD and no specific caspofungin breakpoints. Use anidulafungin and micafungin as markers for caspofungin susceptibility where isolates "S" to both…

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Media Preparation

Media Preparation Media preparation for EUCAST disk diffusion testing and for determination of MIC values by the broth microdilution method Version 7.0, January 2022 Download PDF EUCAST antimicrobial susceptibility testing is based on MH agar and MH broth, without supplements for non-fastidious…

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Subcommittees

Subcommittees EUCAST subcommittees are set up to deal with specific issues or areas requiring particular expertise. Consultations and decisions on subcommittee proposals are made through the EUCAST Steering Committee and follows the structure of all other consultations and decisions. When…

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EDL MIC Testing Services

EDL MIC Testing Services The EUCAST Development Laboratory in Växjö, Sweden offers to determine minimum inhibitory concentrations (MICs) using broth microdilution according to the ISO standard 20776-1 (2019) to offer colleagues high quality MIC determination. The service is primarily organised to…

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Steering Committee

EUCAST Steering Committee The ESCMID-appointed Steering Committee consists of the Chair, Scientific Secretary, Clinical Data Co-ordinator, Technical Data Co-ordinator, Head of the EUCAST Development Laboratory, Educational Officer, Webmaster, Pharmacokinetic/Pharmacodynamic experts, a representative…

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EUCAST Panel of S. pneumoniae and betalactam agents

EUCAST Panel of S. pneumoniae and betalactam agents The panel consists of 10 strains of Streptococcus pneumoniae with reference MIC values (mg/L) representing varying levels of susceptibility to beta-lactam agents, from wild type isolates to highly resistant. However, although the selection of…

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