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General

A revised version of the guidance on "What to do when there are no breakpoints" published

Following frequent questions and pleas for further guidance, EUCAST has revised the document " What to do when there are no breakpoints ". This is not the type of document where a consultation is part of the process. However, it is important that colleagues understand and agree with the advice…

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RAST methodology updated

Rapid AST directly from positive blood culture bottles (RAST) - several documents were updated. the breakpoint table was updated - new information on how to screen for and characterize benzylpenicillin resistance in Streptococcus pneumoniae added. files describing the correlation between RAST zone…

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Disk Diffusion Implementation

Disk Diffusion Implementation Check list to facilitate implementation of antimicrobial susceptibility testing with EUCAST breakpoints Version 1.0, 28 May 2010 Download PDF This document aims to help in the implementation of EUCAST susceptibility testing. The checklist was developed and tried by…

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VetCAST

Veterinary Committee on Antimicrobial Susceptibility Testing (VetCAST) VetCAST is a EUCAST subcommittee dealing with all aspects of antimicrobial susceptibility testing of bacterial pathogens of animal origin and animal bacteria with zoonotic potential. Subcommittee Discover More Development of…

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Methodology and Instructions

Methodology and Instructions Although the general principles for the development of breakpoints are similar to those for bacteria, susceptibility testing of fungi needs special attention. This page lists the documents that address the methods needed for testing of different fungi. AST of Yeasts…

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Colistin susceptibility testing

A few weeks ago, we informed EUCAST users, that the Warning against bioMerieux AST material for colistin, still stands. The item referred (by a link) to the EUCAST Warnings page where it was made clear exactly which material was malfuntioning and should have been withdrawn by bioMerieux. However,…

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ECOFFinder software removed from the EUCAST website

ECOFFinder. The previously available ECOFFinder software is no longer accessible on the EUCAST website because, according to EUCAST procedures ( SOP 10.2), setting ECOFFs or TECOFFs on single or already aggregated distributions is not acceptable according to EUCAST recommendations. Software and…

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Rapid AST in blood culture

Rapid AST in blood culture For selection of an efficacious antibiotic therapy rapid susceptibility results are desirable, but the usual overnight incubation precludes rapid results. EUCAST RAST fills this gap by providing results within 4 to 8 hours of incubation. The method is validated for…

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Rationale Documents

Rationale Documents The EUCAST Rationale Documents currently available are listed below. Rationale documents for antifungal agents are listed elsewhere, see " Antifungal rationale documents ". A template for companies aiming to create a comprehensive rationale document for new agents or for existing…

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Publications and Presentations

Publications and Presentations 2025 2023 2022 2020 Archive The Mycobacterium tuberculosis complex based on the EUCAST reference protocol. Mansjö M, Espinosa-Gongora C, Samanci I, Groenheit R, Werngren J. Antimicrob Agents Chemother. 2025 Feb 13;69(2):e0094624. doi: 10.1128/aac.00946-24. Epub 2024…

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