Susceptibility testing of fastidious organisms - is there an alternative to MH-F ? Public consultation 2025-06-19 - 08-29. The EUCAST medium MH-F was developed for AST of fastidious organisms.Colleagues in some areas of the world have difficulties obtaining the MH-F plate based on horse blood. The…
Get to know moreMethods and Quality Control EUCAST has validated a method for direct plating of disk diffusion MH and MHF agar plates from positive blood culture bottles. The plates are immediately incubated and read after 4, 6, 8 or 16-20 hours (but not beyond) of incubation. Document Link Methodology - EUCAST…
Get to know moreThe database of MIC and zone diameter distributions was created by Gunnar Kahlmeter for EUCAST from 2002 and onwards. More data is regularly added and all data is curated by Gunnar Kahlmeter and John Turnidge. The website gives MIC distributions and inhibition zone diameter distributions for…
Get to know moreEUCAST distributions of MIC and Zone diameters (https:/mic.eucast.org; choose either “MIC” or “Disk diffusion”) are freely available. When sufficiently many acceptable distributions are available to the curators, ECOFFs are determined and listed beneath each graph together with confidence intervals.…
Get to know moreRationale documents Amphotericin B v 3.0 (26 June 2025) Anidulafungin vs. Candida v 5.0 (26 June 2025) Caspofungin vs Candida - there is no caspofungin RD and no specific caspofungin breakpoints. Use anidulafungin and micafungin as markers for caspofungin susceptibility where isolates "S" to both…
Get to know moreThe manufacturer of antimicrobial susceptibility testing material is responsible for the quality of the material provided to microbiological laboratories. The laboratory is responsible for the result provided to doctors and patients. To perform AST where there is intrinsic resistance is wasting the…
Get to know moreDisk diffusion of anaerobic bacteria in 16-20 hours (9 December, 2021; at 8AM , 1PM and 4PM CET to satisfy different time zones; link below). EUCAST has developed a disk diffusion method for AST of 5 anaerobic species (Bacteroides spp, Prevotella spp, Fusobacterium necrophorum, Clostridium…
Get to know moreMedia Preparation Media preparation for EUCAST disk diffusion testing and for determination of MIC values by the broth microdilution method Version 7.0, January 2022 Download PDF EUCAST antimicrobial susceptibility testing is based on MH agar and MH broth, without supplements for non-fastidious…
Get to know moreSubcommittees EUCAST subcommittees are set up to deal with specific issues or areas requiring particular expertise. Consultations and decisions on subcommittee proposals are made through the EUCAST Steering Committee and follows the structure of all other consultations and decisions. When…
Get to know moreFrom EUCAST, we wish you all happy holidays and a better 2021 than the 2020 to which we are now saying goodbye. EUCAST has, despite the pandemic, had a busy year - no travelling but many hours of online meetings and online seminars. There were new agents, new species and a new MIC and new Zone…
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