AST of fungi

Antifungal susceptibility testing (AFST)

The AFST issues a warning (April 2024) against AST methods for amphothericin B testing against Candida auris. Read more on the EUCAST Warnings page.


Breakpoints and methods for susceptibility testing of yeasts, moulds and dermatophytes are developed and validated by the EUCAST subcommittee on AFST.


New and revised documents open for consultation will until accepted be published in the EUCAST News section together with all other consultations from EUCAST.  


Information on subcommittee organisation and members are available on the webpage describing the Organisation of EUCAST.


Information for industry aiming to bring agents to EUCAST for review and revision of breakpoints or a new agent to EMA for registration is available at  Information for industry


Development of new methods and validation and calibration of existing methods is performed at the EUCAST Development Laboratory for AFST: The EUCAST Development Laboratory for Antifungal Susceptibility Testing 


with the help of


The EUCAST AFST Network Laboratories 


For Copyright statement and reference to EUCAST material, see the homepage of EUCAST.


Contacting EUCAST-AFST


Contact the AFST through the EUCAST subject related contact form

Chairman Maiken Cavling Arendrup (maiken.c.arendrup[at]

Scientific Secretary Jesus Guinea Ortega (jesusguineaortega[at]

Clinical Data Coordinator Joseph Meletiadis (joseph.meletiadis[at]



EUCAST Development Laboratory for fungi

c/o Unit of Mycology

Dept. Microbiology & Infection Control

Statens Serum Institut, Building 211

Artillerivej 5

DK-2300 Copenhagen


Phone: +45 3268 3223 or +45 3268 3116