Rapid AST in bloodcultures

RAST from EUCAST in publications

EUCAST RAST is implemented widely. An increasing number of publications are available. Via library searches for "EUCAST" and "EUCAST RAST" we have identified an array of publications. They are presented in reverse chronological order.


1. The EUCAST rapid disc diffusion method for antimicrobial susceptibility testing

directly from positive blood culture bottles. 

Jonasson E, Matuschek E, Kahlmeter G.

J Antimicrob Chemother. 2020 Apr 1;75(4):968-978. doi: 10.1093/jac/dkz548.PMID: 32016342 


2. EUCAST rapid antimicrobial susceptibility testing (RAST) in blood cultures:

validation in 55 European laboratories. 

Åkerlund A, Jonasson E, Matuschek E, Serrander L, Sundqvist M, Kahlmeter G; RAST Study Group.

J Antimicrob Chemother. 2020 Nov 1;75(11):3230-3238. doi: 10.1093/jac/dkaa333.PMID: 32789506 


3. Evaluation of prolonged incubation time of 16-20 h with the EUCAST rapid

antimicrobial susceptibility disc diffusion testing method. 

Jonasson E, Matuschek E, Kahlmeter G, 

J Antimicrob Chemother. 2023 Dec 1;78(12):2926-2932. doi: 10.1093/jac/dkad332. PMID: 37864550


4. Fully Automated EUCAST Rapid Antimicrobial Susceptibility Testing (RAST)

from Positive Blood Cultures: Diagnostic Accuracy and Implementation.

Abdessalam Cherkaoui, D. Schorderet, N. Azam, L. Crudeli, J. Fernandez, G. Renzi, A. Fischer, J. Schrenzel. 

J Clin Microbiol 2022 Oct 19;60(10):e0089822. doi: 10.1128/jcm.00898-22. Epub 2022 Sep 29.


5. EUCAST rapid antimicrobial susceptibility testing (RAST): analytical

performance and impact on patient management.

Benjamin Berinson, Flaminia Olearo, Anna Both, Nicole Brossmann, Martin Christner, Martin Aepfelbacher, Holger Rohde. 

J Antimicrob Chemother, Volume 76, Issue 5, May 2021, Pages 1332–1338, https://doi.org/10.1093/jac/dkab026 


6. Multicentre Evaluation of the EUCAST Rapid Antimicrobial Susceptibility

Testing (RAST) ­Extending Analysis to 16-20 Hours Reading Time

Gabriele Bianco, D. Lombardo, G. Ricciardelli, M. Boattini, S. Comini, R. Cavallo, C. Costa, S. Ambretti. 

Antibiotics (Basel) 2022 Oct 13;11(10):1404.  doi: 10.3390/antibiotics11101404.


7. Analytical Performance and Potential Clinical Utility of EUCAST Rapid

Antimicrobial Susceptibility Testing in Blood Cultures after Four Hours of Incubation.

Anna Ekwall-Larson, Inga Fröding, Berivan Mert, Anna Åkerlund, Volkan Özenci. 

Microbiol Spectr. 2023 Feb 21;11(2):e0500122. doi: 10.1128/spectrum.05001-22. doi: 10.1128/spectrum.05001-22


8. Validation of Rapid Antimicrobial Susceptibility Testing directly from blood

cultures using WASPLab®, including Colibrí™ and Radian® in-Line Carousel. 

Herroelen PH, Heestermans R, Emmerechts K, Vandoorslaer K, Wybo I, Piérard D, Muyldermans A.

Eur J Clin Microbiol Infect Dis. 2022 Jun;41(6):1001. doi: 10.1007/s10096-022-04451-2.PMID: 35511407


9. Evaluation of the EUCAST Rapid Antimicrobial Susceptibility Test for 

Enterobacterales-Containing Blood Cultures in China.

Shan Y, Hu B, Guo W, Wang B, Zhou C, Huang S, Li N.

J Clin Microbiol. 2022 Apr 20;60(4):e0255921. doi: 10.1128/jcm.02559-21


10. Impact of EUCAST rapid antimicrobial susceptibility testing (RAST)

on management of Gram-negative bloodstream infection. 

Cardot Martin E, Colombier MA, Limousin L, Daude O, Izarn O, Cahen P, Farfour E, Lesprit P, Vasse M.

Infect Dis Now. 2022 Nov;52(8):421-425. doi: 10.1016/j.idnow.2022.09.002. Epub 2022 Sep 13. PMID: 36108973


11. Usefulness of EUCAST rapid antibiotic susceptibility breakpoints and screening cut-off

values directly from blood cultures for the inference of β-lactam resistance mechanisms in Enterobacterales. 

Cerrudo V, Cortés-Cuevas JL, García-Fernández S, Morosini MI, Cantón R, Sánchez-Díaz AM.

JAC Antimicrob Resist. 2023 Feb 17;5(1):dlad017. doi: 10.1093/jacamr/dlad017. eCollection 2023 Feb. PMID: 36816745 


12. EUCAST rapid antimicrobial susceptibility testing (RAST) compared to conventional

susceptibility testing: implementation and potential added value in a tertiary hospital in Belgium. 

Strubbe G, Messiaen AS, Vandendriessche S, Verhasselt B, Boelens.

J. Acta Clin Belg. 2023 Oct;78(5):385-391. doi: 10.1080/17843286.2023.2197314. Epub 2023 Mar 31.PMID: 36999562