Rapid AST in bloodcultures

Method - Rapid AST directly from positive blood culture bottles

EUCAST has validated a method for direct plating of disk diffusion MH and MHF agar plates for reading after 4, 6, 8 and 16-20 hours (but not beyond) of incubation.

Currently the method is validated for E. coli, K.pneumoniae, Ps. aeruginosa, Acinetobacter baumannii, S. aureus, E.faecalis, E.faecium and S.pneumoniae and for a limited panel of antimicrobials.
Work is ongoing to extend the method to more species and more antibiotics. 

For methodology, see the document EUCAST Rapid AST directly from positive blood culture bottles (vs. 3, 12 April, 2022) 

For hints on how to implement the rapid AST in the laboratory, see Implementation of RAST (updated 12 April, 2022).

 

 

Archived versions:

EUCAST rapid AST directly from positive blood culture bottles (valid until 12 April, 2022) 

EUCAST rapid AST directly from positive blood culture bottles (valid until 31 December, 2021

EUCAST rapid AST directly from positive blood culture bottles (valid until 2 May, 2019)